anti p egfr y1068  (R&D Systems)

Journal: Theranostics

Article Title: In situ single-cell therapeutic response imaging facilitated by the TRIPODD fluorescence imaging platform

doi: 10.7150/thno.93256

Figure Lengend Snippet: Validated antibody panel for multiplex cyCIF imaging.

Article Snippet: A primary antibody reactive to human phospho-EGFR (CST, pEGFR) was purchased and used unconjugated for indirect immunofluorescence with a donkey anti-rabbit secondary (dRb) antibody conjugated to Cyanine-7 (Cy7).

Techniques: Multiplex Assay, Imaging Biomarker, Imaging

Journal: Theranostics

Article Title: In situ single-cell therapeutic response imaging facilitated by the TRIPODD fluorescence imaging platform

doi: 10.7150/thno.93256

Figure Lengend Snippet: Pearson's correlation of iPAI signal to EGFR expression and pharmacodynamic assessment of iPAI probes in EGFR-positive xenografts. Pearson correlation coefficients were calculated to compare EGFR expression to A. DTA, B. targeted probe and C. untargeted probe mean fluorescence intensity (MFI). Pearson correlation coefficients were also calculated to study the relationship between pEGFR expression and D. DTA, E. targeted probe and F. untargeted probe MFI. Solid lines represent the linear regression trendline and are the color of their respective dose group. Calculated correlation coefficients per dose group are also color coded using the same scheme. HCC827 and PANC-1 CDX bearing mice were injected with either vehicle, 1.25 mg/kg, 2.5 mg/kg, or 5 mg/kg of iPAI probes and G. targeted probe fluorescence intensity, H. untargeted fluorescence intensity and I. DTA were measured. Significance in all plots is denoted by the number of asterisks as follows: *p < 0.05, **p < 0.01 and ***p < 0.001.

Article Snippet: A primary antibody reactive to human phospho-EGFR (CST, pEGFR) was purchased and used unconjugated for indirect immunofluorescence with a donkey anti-rabbit secondary (dRb) antibody conjugated to Cyanine-7 (Cy7).

Techniques: Expressing, Fluorescence, Injection

Journal: Theranostics

Article Title: In situ single-cell therapeutic response imaging facilitated by the TRIPODD fluorescence imaging platform

doi: 10.7150/thno.93256

Figure Lengend Snippet: Single-cell quantification of erlotinib therapeutic response and DTA. The single-cell z-scored expressions for each biomarker in each treatment cohort were combined to compare the control, untreated cohort (NoTx) to the erlotinib therapeutic response measured in the erlotinib treated cohorts (i.e., 6h, 12h or 24h). The signal levels of A. EGFR, B. pEGFR, C. DTA, D. Akt, E. pAkt, F. Ki67, G. MEK, H. pMEK and I. CC3 were quantified for cohort comparison.

Article Snippet: A primary antibody reactive to human phospho-EGFR (CST, pEGFR) was purchased and used unconjugated for indirect immunofluorescence with a donkey anti-rabbit secondary (dRb) antibody conjugated to Cyanine-7 (Cy7).

Techniques: Clinical Proteomics, Biomarker Discovery, Control, Comparison